siRNA/ASO Synthesis

High Purity and Consistency for Any siRNA/ASO
Advanced AI Design Ensures Higher Knockdown Efficiency

Overview Service Details Case Studies Resources Contact Us

Home » Oligo Synthesis Applications » Small Nucleic Acid Drugs Synthesis

Overview

With over 22 years of expertise, GenScript provides comprehensive siRNA and ASO synthesis services, from R&D to cGMP production, to support small RNA drug development.

Advantages

Superior quality,
delivered in 4 days

siRNA/ASO products with ≥90% purity and excellent consistency,
ensuring superior knockdown efficiency

AI-Enhanced Precision Design

AI combined with experimental validation optimizes design logic,
achieving knockdown efficiency up to 98%

Proprietary modifications

Boost drug development with enhanced stability and reduced off-target effects

Need help from an expert?

Book A Call

Email Us

Special Recommendation

siRNA Guarantee Package: AI-Enhanced Precision Design, ensuring knockdown efficiency ≥80%

Oligo Synthesis

Service Details

Service	Length	Quantity	Type	Purification	Modification	Conjugation
siRNA	20-30nt	5 nmol to Kg level	Multi- transcripts High-throughput siRNA Cross-species siRNA	RNase free HPLC, desalt	Locked Nucleic Acid (LNA), thioate, methylation, 2-Ome etc. (~200 types)	Antibody, peptide, lipid etc.
ASO	15-30nt	5 nmol to Kg level	Occupancy mechanism Degradation mechanism	RNase free HPLC, desalt		Antibody, peptide, lipid etc.

* QC and Specifications >>

QC Item	Test Method	Specification
Appearance	Visual Inspect	White to pale yellow powder
Identity by MW	Mass Spectrometry	Theoretical Average MW ± 0.1%
LC-MS	Liquid chromatography–mass spectrometry	Report result
LC MS/MS Sequencing	High-resolution Mass Spectrometry Sequencing	100% sequence coverage
Purity by HPLC	High Performance Liquid Chromatography	double-strand siRNA ≥ 90%
Purity by CGE	Capillary Gel Electrophoresis	Report result
Purity by PAGE	Polyacrylamide Gel Electrophoresis	Report result
pH Measurement	Micro pH Electrode Method	Report result
TEA Salt residue detection	Gas chromatography-mass spectrometry Measurement	Report result
Solvent residue	Gas chromatography-mass spectrometry Measurement	Report result
Endotoxin	Chromogenic Method	Report result
Bacterial Testing	Direct Inoculation /membrane filter method	Report result

Please find more customized QC information in Oligo Quality Control Service>>.

cGMP RNAi Oligonucleotides Request Form>>

Online Ordering

Request Now

Design Tools

siRNA Design Tool

siRNA Negative Control (Scramble sequence) Design Tool

* GenScript offers a free siRNA design tool utilizing classic design logic. If you prefer an AI-enhanced design platform, please choose the siRNA guarantee package or send a request to oligo@genscript.com.

Case Studies

Accurate MW, high purity and batch-to-batch consistency
Highly-modified siRNA with purity up to 96.55%

20 nt double-stranded siRNA with 29 2'-O-Methyl modifications, 3 phosphate groups, 13 2'-Fluoro modifications, and 1 GalNAc modification, exhibited purity up to 96.55%
Advanced AI design ensures higher knockdown efficiency

Genscript's Small Nucleic Acid Drugs AI Design Platform

siRNA sequences designed for Gene B with GenScript's bioinformatic design platform achieved higher knockdown efficiency (KD%) than those created with other vendors' tools.
Proprietary modifications significantly enhance siRNA stability
Method: For different modification schemes, the metabolic rate and metabolites in a serum environment were analyzed to select the most stable modification schemes for subsequent optimization and development.

Results
- siRNAs with modifications from groups GS1 to GS6 exhibited lower degradation levels and better stability compared to unmodified siRNA (sense strand SS/antisense strand AS).
- Among these, the GS6 modification scheme demonstrated the lowest degradation level and the highest stability, making it the most optimal modification scheme.